S4-S5 linker movement during activation and inactivation in voltage-gated K⁺ channels.

ABSTRACT: The S4-S5 linker physically links voltage sensor and pore domain in voltage-gated ion channels and is essential for electromechanical coupling between both domains. Little dynamic information is available on the movement of the cytosolic S4-S5 linker due to lack of a direct electrical or optical readout. To understand the movements of the gating machinery during activation and inactivation, we incorporated fluorescent unnatural amino acids at four positions along the linker of the Shaker K_V channel. Using two-color voltage-clamp fluorometry, we compared S4-S5 linker movements with charge displacement, S4 movement, and pore opening. We found that the proximal S4-S5 linker moves with the S4 helix throughout the gating process, whereas the distal portion undergoes a separate motion

SUBMITTER: Kalstrup T 

PROVIDER: S-EPMC6055142 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature