Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation.

ABSTRACT: The ability to control the activity of CRISPR-dCas9 with precise spatiotemporal resolution will enable tight genome regulation of user-defined endogenous genes for studying the dynamics of transcriptional regulation. Optogenetic devices with minimal phototoxicity and the capacity for deep tissue penetration are extremely useful for precise spatiotemporal control of cellular behavior and for future clinic translational research. Therefore, capitalizing on synthetic biology and optogenetic design principles, we engineered a far-red light (FRL)-activated CRISPR-dCas9 effector (FACE) device that induces transcription of exogenous or endogenous genes in the presence of FRL stimulation. This versatile system provides a robust and convenient method for precise spatiotemporal control of endogenous

SUBMITTER: Shao J 

PROVIDER: S-EPMC6055150 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature