Optic nerve as a source of activated retinal microglia post-injury.

ABSTRACT: Using mice expressing green fluorescent protein (GFP) from a transgenic CD11c promoter we found that a controlled optic nerve crush (ONC) injury attracted GFP^hi retinal myeloid cells to the dying retinal ganglion cells and their axons. However, the origin of these retinal myeloid cells was uncertain. In this study we use transgenic mice in conjunction with ONC, partial and full optic nerve transection (ONT), and parabiosis to determine the origin of injury induced retinal myeloid cells. Analysis of parabiotic mice and fate mapping showed that responding retinal myeloid cells were not derived from circulating macrophages and that GFP^hi myeloid cells could be derived from GFP^lo microglia. Comparison of optic nerve to retina following an ONC showed a much grea

SUBMITTER: Heuss ND 

PROVIDER: S-EPMC6055350 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature