A whole blood model of thrombocytopenia that controls platelet count and hematocrit.

ABSTRACT: In patients with thrombocytopenia, it can be difficult to predict a patient's bleeding risk based on platelet count alone. Platelet reactivity may provide additional information; however, current clinical assays cannot reliably assess platelet function in the setting of thrombocytopenia. New methods to study platelet reactivity in thrombocytopenic samples are needed. In this study, we sought to develop a laboratory model of thrombocytopenia using blood from healthy subjects that preserves the whole blood environment and reproducibly produces samples with a specific platelet count and hematocrit. We compared the activation state of unstimulated and agonist-stimulated platelets in thrombocytopenic samples derived from this method with normocytic controls. Whole blood was diluted with autolog

SUBMITTER: Bercovitz RS 

PROVIDER: S-EPMC6055520 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature