Isolation and characterization of microsatellite loci from Oxytropis diversifolia (Fabaceae).

ABSTRACT:

Premise of the study

Microsatellite primers were developed for a perennial legume from northern China, Oxytropis diversifolia (Fabaceae), to investigate population genetic structure of this taxon, as well as potential hybridization events with closely related taxa in this genus.

Methods and results

One hundred and five primer pairs were designed from Illumina sequence data and screened for suitability. Fifteen of these primer pairs were polymorphic, and these primers amplified tri-, tetra-, and pentanucleotide repeats with 10-56 alleles per locus. Cross-amplification tests in three other Oxytropis species from northern China (O. leptophylla, O. neimonggolica, and O. squammulosa) revealed that all of these loci can be amplified successfully

SUBMITTER: Wang H 

PROVIDER: S-EPMC6055551 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature