Generating DNA sequence data with limited resources for molecular biology: Lessons from a barcoding project in Indonesia.

ABSTRACT: The advent of the DNA sequencing age has led to a revolution in biology. The rapid and cost-effective generation of high-quality sequence data has transformed many fields, including those focused on discovering species and surveying biodiversity, monitoring movement of biological materials, forensic biology, and disease diagnostics. There is a need to build capacity to generate useful sequence data in countries with limited historical access to laboratory resources, so that researchers can benefit from the advantages offered by these data. Commonly used molecular techniques such as DNA extraction, PCR, and DNA sequencing are within the reach of small laboratories in many countries, with the main obstacles to successful implementation being lack of funding and limited practical experience.

SUBMITTER: Dean GH 

PROVIDER: S-EPMC6055555 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature