Capturing variation in Lens (Fabaceae): Development and utility of an exome capture array for lentil.

ABSTRACT:

Premise of the study

Lentil is an important legume crop with reduced genetic diversity caused by domestication bottlenecks. Due to its large and complex genome, tools for reduced representation sequencing are needed. We developed an exome capture array for use in various genetic diversity studies.

Methods

Based on the CDC Redberry draft genome, we developed an exome capture array using multiple sources of transcript resources. The probes were designed to target not only the cultivated lentil, but also wild species. We assessed the utility of the developed method by applying the generated data set to population structure and phylogenetic analyses.

Results

The data set includes 16 wild lentils and 22 cultivar accessions of lentil. Alignment rates were over 90%, and the

SUBMITTER: Ogutcen E 

PROVIDER: S-EPMC6055568 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature