Widespread epigenomic, transcriptomic and proteomic differences between hip osteophytic and articular chondrocytes in osteoarthritis.

ABSTRACT:

Objectives

To identify molecular differences between chondrocytes from osteophytic and articular cartilage tissue from OA patients.

Methods

We investigated genes and pathways by combining genome-wide DNA methylation, RNA sequencing and quantitative proteomics in isolated primary chondrocytes from the cartilaginous layer of osteophytes and matched areas of low- and high-grade articular cartilage across nine patients with OA undergoing hip replacement surgery.

Results

Chondrocytes from osteophytic cartilage showed widespread differences to low-grade articular cartilage chondrocytes. These differences were similar to, but more pronounced than, differences between chondrocytes from osteophytic and high-grade articular cartilage, and more pronounced than differences betwee

SUBMITTER: Steinberg J 

PROVIDER: S-EPMC6055583 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature