In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography.

ABSTRACT: Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real-time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well-established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated t

SUBMITTER: Molenaar SD 

PROVIDER: S-EPMC6055872 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature