Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation.

ABSTRACT: Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viru

SUBMITTER: Costantini V 

PROVIDER: S-EPMC6056096 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature