Subtle changes in light intensity affect in vitro responses but not ex vitro performance of Limonium sinuatum.

ABSTRACT: Environmental conditions during the in vitro stages of a micropropagation process can further affect the ex vitro plant performance. In this work, the behaviour of four blue cultivars of statice (Limonium sinuatum) was studied with respect to four distances (18.0, 12.5, 8.0, or 2.0 cm) between culture tubes and the light source during their in vitro multiplication stage. In vitro shoots of statice were cultured on MS medium supplemented with 4% sucrose, 0.9% agar and 0.2 mg L^-1 BA and incubated under four slightly different light intensity treatments (PPFD of 96.9, 99.6, 101.2, or 102.7 µmol m^-2 s^-1) for 35 days at 23 °C with a daily photoperiod of 16 h. The light treatments assayed resulted in no significant differences in in vitro propagation ratio

SUBMITTER: Sanchez-Sanchez J 

PROVIDER: S-EPMC6056352 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature