Novel Butane-Oxidizing Bacteria and Diversity of bmoX Genes in Puguang Gas Field.

ABSTRACT: To investigate the diversity of butane-oxidizing bacteria in soils contaminated by long-term light hydrocarbon microseepage and the influence of butane on the soil microbial community, a quantitative study and identification of butane-oxidizing bacteria (BOB) in soils at the Puguang gas field were performed by DNA-based stable isotope probing (DNA-SIP). For the first time, two phylotypes corresponding to the genera Giesbergeria and Ramlibacter were identified as being directly involved in butane oxidation, in addition to the well-known light hydrocarbon degrader Pseudomonas. Furthermore, bmoX genes were strongly labeled by ¹³C-butane, and their abundances in gas field soils increased by 43.14-, 17.39-, 21.74-, and 30.14-fold when incubated with butane

SUBMITTER: Deng Y 

PROVIDER: S-EPMC6056644 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature