Neuronal Growth Cone Size-Dependent and -Independent Parameters of Microtubule Polymerization.

ABSTRACT: Migration and pathfinding of neuronal growth cones during neurite extension is critically dependent on dynamic microtubules. In this study we sought to determine, which aspects of microtubule polymerization relate to growth cone morphology and migratory characteristics. We conducted a multiscale quantitative microscopy analysis using automated tracking of microtubule plus ends in migrating growth cones of cultured murine dorsal root ganglion (DRG) neurons. Notably, this comprehensive analysis failed to identify any changes in microtubule polymerization parameters that were specifically associated with spontaneous extension vs. retraction of growth cones. This suggests that microtubule dynamicity is a basic mechanism that does not determine the polarity of growth cone response but can be ex

SUBMITTER: Kiss A 

PROVIDER: S-EPMC6056669 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature