NMR Metabolomics Defining Genetic Variation in Pea Seed Metabolites.

ABSTRACT: Nuclear magnetic resonance (NMR) spectroscopy profiling was used to provide an unbiased assessment of changes to the metabolite composition of seeds and to define genetic variation for a range of pea seed metabolites. Mature seeds from recombinant inbred lines, derived from three mapping populations for which there is substantial genetic marker linkage information, were grown in two environments/years and analyzed by non-targeted NMR. Adaptive binning of the NMR metabolite data, followed by analysis of quantitative variation among lines for individual bins, identified the main genomic regions determining this metabolic variability and the variability for selected compounds was investigated. Analysis by t-tests identified a set of bins with highly significant associations to genetic

SUBMITTER: Ellis N 

PROVIDER: S-EPMC6056766 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature