Group IIA-Secreted Phospholipase A₂ in Human Serum Kills Commensal but Not Clinical Enterococcus faecium Isolates.

ABSTRACT: Human innate immunity employs cellular and humoral mechanisms to facilitate rapid killing of invading bacteria. The direct killing of bacteria by human serum is attributed mainly to the activity of the complement system, which forms pores in Gram-negative bacteria. Although Gram-positive bacteria are considered resistant to killing by serum, we uncover here that normal human serum effectively kills Enterococcus faecium Comparison of a well-characterized collection of commensal and clinical E. faecium isolates revealed that human serum specifically kills commensal E. faecium strains isolated from normal gut microbiota but not clinical isolates. Inhibitor studies show that the human group IIA secreted phospholipase A2 (hGIIA), but not complement, is responsible for killi

SUBMITTER: Paganelli FL 

PROVIDER: S-EPMC6056852 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature