The agr quorum sensing system in Staphylococcus aureus cells mediates death of sub-population.

ABSTRACT:

Objective

In the human pathogen, Staphylococcus aureus, the agr quorum sensing system controls expression of a multitude of virulence factors and yet, agr negative cells frequently arise both in the laboratory and in some infections. The aim of this study was to examine the possible reasons behind this phenomenon.

Results

We examined viability of wild type and agr mutant cell cultures using a live-dead stain and observed that in stationary phase, 3% of the wild type population became non-viable whereas for agr mutant cells non-viable cells were barely detectable. The effect appears to be mediated by RNAIII, the effector molecule of agr, as ectopic overexpression of RNAIII resulted in 60% of the population becoming non-viable. This effect was not due to toxicity from delta to

SUBMITTER: Paulander W 

PROVIDER: S-EPMC6057012 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature