Detection of parvovirus B19 in selected high-risk patient groups & their phylogenetic & selection analysis.

ABSTRACT:

Background & objectives

Human parvovirus B19V (B19V) is known to be associated with erythema infectiosum commonly in children, aplastic crisis, especially in persons with underlying haemolytic disorders, hydrops fetalis in pregnancies and arthritis. This cross-sectional study was aimed to determine the presence of B19V infection in childhood febrile illnesses, association of B19V with arthropathies and in adult patients with end-stage renal disease (ESRD) on dialysis. The genetic diversity among the sequences was also analysed.

Methods

A nested polymerase chain reaction (nPCR) assay was used for B19V DNA targeting VP1/VP2 region and used for testing 618 patients and 100 healthy controls. Phylogenetic analysis on nucleotide and amino acid sequences was carried out to compare

SUBMITTER: Vadivel K 

PROVIDER: S-EPMC6057248 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature