Exploratory re-encoding of yellow fever virus genome: new insights for the design of live-attenuated viruses.

ABSTRACT: Virus attenuation by genome re-encoding is a pioneering approach for generating effective live-attenuated vaccine candidates. Its core principle is to introduce a large number of synonymous substitutions into the viral genome to produce stable attenuation of the targeted virus. Introduction of large numbers of mutations has also been shown to maintain stability of the attenuated phenotype by lowering the risk of reversion and recombination of re-encoded genomes. Identifying mutations with low fitness cost is pivotal as this increases the number that can be introduced and generates more stable and attenuated viruses. Here, we sought to identify mutations with low deleterious impact on the in vivo replication and virulence of yellow fever virus (YFV). Following comparative bioinformat

SUBMITTER: Klitting R 

PROVIDER: S-EPMC6057501 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature