Stabilization of Bacillus subtilis Spx under cell wall stress requires the anti-adaptor protein YirB.

ABSTRACT: Spx is a global transcriptional regulator present in low-GC Gram-positive bacteria, including the model bacterium Bacillus subtilis and various human pathogens. In B. subtilis, activation of Spx occurs in response to disulfide stress. We recently reported, however, that induction of Spx also occurs in response to cell wall stress, and that the molecular events that result in its activation under both stress conditions are mechanistically different. Here, we demonstrate that, in addition to up-regulation of spx transcription through the alternative sigma factor σM, full and timely activation of Spx-regulated genes by cell wall stress requires Spx stabilization by the anti-adaptor protein YirB. YirB is itself transcriptionally induced under cell wall stress, but not disulfide stress, and thi

SUBMITTER: Rojas-Tapias DF 

PROVIDER: S-EPMC6057675 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature