Crystal-on-crystal chips for in situ serial diffraction at room temperature.

ABSTRACT: Recent developments in serial crystallography at X-ray free electron lasers (XFELs) and synchrotrons have been driven by two scientific goals in structural biology - first, static structure determination from nano or microcrystals of membrane proteins and large complexes that are difficult for conventional cryocrystallography, and second, direct observations of transient structural species in biochemical reactions at near atomic resolution. Since room-temperature diffraction experiments naturally demand a large quantity of purified protein, sample economy is critically important for all steps of serial crystallography from crystallization, crystal delivery to data collection. Here we report the development and applications of "crystal-on-crystal" devices to facilitate large-scale in situ s

SUBMITTER: Ren Z 

PROVIDER: S-EPMC6057835 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature