Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis.

ABSTRACT:

Background and purpose

The cannabinoid system exerts functional regulation of neural stem cell (NSC) proliferation and adult neurogenesis, yet not all effects of cannabinoid-like compounds seen can be attributed to the cannabinoid 1 (CB₁ ) or CB₂ receptor. The recently de-orphaned GPR55 has been shown to be activated by numerous cannabinoid ligands suggesting that GPR55 is a third cannabinoid receptor. Here, we examined the role of GPR55 activation in NSC proliferation and early adult neurogenesis.

Experimental approach

The effects of GPR55 agonists (LPI, O-1602, ML184) on human (h) NSC proliferation in vitro were assessed by flow cytometry. Human NSC differentiation was determined by flow cytometry, qPCR and immunohistochemistry. Immature neuron format

SUBMITTER: Hill JD 

PROVIDER: S-EPMC6057896 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature