Defining Nutrient Combinations for Optimal Growth and Polyhydroxybutyrate Production by Methylosinus trichosporium OB3b Using Response Surface Methodology.

ABSTRACT: Methane and methanol are common industrial by-products that can be used as feedstocks for the production of value-added products by methylotrophic bacteria. Alphaproteobacterial methanotrophs are known to produce and accumulate the biopolymer polyhydroxybutyrate (PHB) under conditions of nutrient starvation. The present study determined optimal production of biomass and PHB by Methylosinus trichosporium OB3b as a function of carbon source (methane or methanol), nitrogen source (ammonium or nitrate), and nitrogen-to-carbon ratio during growth. Statistical regression analysis with interactions was performed to assess the importance of each factor, and their respective interactions, on biomass and PHB production. Higher biomass concentrations were obtained with methane as carbon source

SUBMITTER: Zaldivar Carrillo JA 

PROVIDER: S-EPMC6058025 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature