LPS Induces mTORC1 and mTORC2 Activation During Monocyte Adhesion.

ABSTRACT: Monocyte adhesion is a crucial step in transmigration and can be induced by lipopolysaccharide (LPS). Here, we studied the role of mammalian target of rapamycin (mTOR) complexes, mTORC1 and mTORC2, and PKC in this process. We used THP-1 cells, a human monocytic cell line, to investigate monocyte adhesion under static and flow conditions. We observed that 1.0 μg/mL LPS increased PI3K/mTORC2 pathway and PKC activity after 1 h of incubation. WYE-354 10^-6 M (mTORC2/mTORC1 inhibitor) and 10^-6 M wortmannin avoided monocyte adhesion in culture plates. In addition, WYE also blocked LPS-induced CD11a expression. Interestingly, rapamycin and WYE-354 blocked both LPS-induced monocyte adhesion in a cell monolayer and actin cytoskeleton rearrangement, confirming mTORC1 involvement

SUBMITTER: Ribeiro MC 

PROVIDER: S-EPMC6058081 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature