Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus.

ABSTRACT:

Background

Low ethanol tolerance of Kluyveromyces marxianus limits its application in high-temperature ethanol fermentation. As a complex phenotype, ethanol tolerance involves synergistic actions of many genes that are widely distributed throughout the genome, thereby being difficult to engineer. TATA-binding protein is the most common target of global transcription machinery engineering for improvement of complex phenotypes.

Results

A random mutagenesis library of K. marxianus TATA-binding protein Spt15 was constructed and subjected to screening under ethanol stress. Two mutant strains with improved ethanol tolerance were identified, one of which (denoted as M2) exhibited increased ethanol productivity. The mutant of Spt15 in strain M2 (denoted as Spt15-M2) ha

SUBMITTER: Li P 

PROVIDER: S-EPMC6058363 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature