The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail.

ABSTRACT:

Background

The discovery of enzymes named lytic polysaccharide monooxygenases (LPMOs) has had a major impact on the efficiency of current commercial cellulase cocktails for saccharification of lignocellulosic biomass. However, the notion that LPMOs use molecular oxygen as a co-substrate and require two externally delivered electrons per catalytic cycle poses a challenge in the development of efficient large-scale industrial processes. Building on the recent discovery that H₂O₂, rather than O₂, is the co-substrate of LPMOs, we show here how cellulose degradation by the LPMO-containing commercial cellulase cocktail Cellic^® CTec2 can be controlled and boosted by supplying the reaction with H₂O₂.

Results

The contr

SUBMITTER: Muller G 

PROVIDER: S-EPMC6058378 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature