Highly sensitive MYD88^L265P mutation detection by droplet digital polymerase chain reaction in Waldenstrom macroglobulinemia.

ABSTRACT: We here describe a novel method for MYD88^L265P mutation detection and minimal residual disease monitoring in Waldenström macroglobulinemia, by droplet digital polymerase chain reaction, in bone marrow and peripheral blood cells, as well as in circulating cell-free DNA. Our method shows a sensitivity of 5.00×10^-5, which is far superior to the widely used allele-specific polymerase chain reaction (1.00×10^-3). Overall, 291 unsorted samples from 148 patients (133 with Waldenström macroglobulinemia, 11 with IgG lymphoplasmacytic lymphoma and 4 with IgM monoclonal gammopathy of undetermined significance) were analyzed: 194 were baseline samples and 97 were followup samples. One hundred and twenty-two of 128 (95.3%) bone marrow and 47/66 (71.2%) baseline pe

SUBMITTER: Drandi D 

PROVIDER: S-EPMC6058774 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature