Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform.

ABSTRACT: Studies that rely on fluorescence imaging of nonadherent cells that are cultured in suspension, such as Escherichia coli, are often hampered by trade-offs that must be made between data throughput and imaging resolution. We developed a platform for microfluidics-assisted cell screening (MACS) that overcomes this trade-off by temporarily immobilizing suspension cells within a microfluidics chip. This enables high-throughput and automated single-cell microscopy for a wide range of cell types and sizes. As cells can be rapidly sampled directly from a suspension culture, MACS bypasses the need for sample preparation, and therefore allows measurements without perturbing the native cell physiology. The setup can also be integrated with complex growth chambers, and can be used to enrich or sort t

SUBMITTER: Okumus B 

PROVIDER: S-EPMC6059380 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature