Cardiac regenerative capacity is age- and disease-dependent in childhood heart disease.

ABSTRACT:

Objective

We sought to define the intrinsic stem cell capacity in pediatric heart lesions, and the effects of diagnosis and of age, in order to inform evidence-based use of potential autologous stem cell sources for regenerative medicine therapy.

Methods

Ventricular explants derived from patients with hypoplastic left heart syndrome (HLHS), tetralogy of Fallot (TF), dilated cardiomyopathy (DCM) and ventricular septal defect (VSD) were analyzed following standard in vitro culture conditions, which yielded cardiospheres (C-spheres), indicative of endogenous stem cell capacity. C-sphere counts generated per 5 mm3 tissue explant and the presence of cardiac progenitor cells were correlated to patient age, diagnosis and echocardiographic function.

Results

Cardiac explants f

SUBMITTER: Traister A 

PROVIDER: S-EPMC6059427 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature