Constructing a DNA barcode reference library for southern herbs in China: A resource for authentication of southern Chinese medicine.

ABSTRACT: Southern Chinese Medicine (SCM) is an important sect of Traditional Chinese Medicine (TCM) with its own special cultural style. Species identification is essential for TCM quality control because authentic herbs are possibly substituted with adulterants that would threaten the health of the public or even cause death. Here, we provided the first local reference DNA barcode library based on the second internal transcribed spacer (ITS2) for the molecular identification of SCM. A total of 1512 specimens of southern herbs representing 359 species were collected under the instructions and identification of taxonomic experts. Genomic DNA was extracted, and the PCR reaction proceeded according to standard procedures. After Sanger sequencing, sequence assembling and annotation, a reliable ITS2 bar

SUBMITTER: Gong L 

PROVIDER: S-EPMC6059470 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature