Correction-free remotely scanned two-photon in vivo mouse retinal imaging.

ABSTRACT: Non-invasive fluorescence retinal imaging in small animals is an important requirement for an array of translational vision applications. The in vivo two-photon imaging of the mouse retina may enable the long-term investigation of the structure and function of healthy and diseased retinal tissue. However, to date, this has only been possible using relatively complex adaptive-optics systems. Here, the optical modeling of the murine eye and of the imaging system is used to achieve correction-free two-photon microscopy through the pupil of a mouse eye to yield high-quality, optically sectioned fundus images. By remotely scanning the focus using an electronically tunable lens, high-resolution three-dimensional fluorescein angiograms and cellular-scale images are acquired, thus introduci

SUBMITTER: Bar-Noam AS 

PROVIDER: S-EPMC6059848 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature