Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators.

ABSTRACT: Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience. We developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains. It can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm³ with respective spatial resolution of 35-200 μm. Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate, for the first time, the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scatter

SUBMITTER: Dean-Ben XL 

PROVIDER: S-EPMC6059886 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature