A Simple Dynamic Strategy to Deliver Stem Cells to Decellularized Nerve Allografts.

ABSTRACT:

Background

The addition of adipose-derived mesenchymal stromal cells to decellularized nerve allografts may improve outcomes of nerve reconstruction. Prior techniques used for cell seeding are traumatic to both the mesenchymal stromal cells and nerve graft. An adequate, reliable, and validated cell seeding technique is an essential step for evaluating the translational utility of mesenchymal stromal cell-enhanced decellularized nerve grafts. The purpose of this study was to develop a simple seeding strategy with an optimal seeding duration.

Methods

A dynamic bioreactor was used to seed rat and human mesenchymal stromal cells separately onto rat and human decellularized nerve allografts. Cell viability was evaluated by MTS assays and cellular topology after seeding was determ

SUBMITTER: Rbia N 

PROVIDER: S-EPMC6059981 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature