Autophagic cell death participates in POMC-induced melanoma suppression.

ABSTRACT: Hypoxia in tumors is known to trigger the pro-survival pathways such as autophagy. Systemic proopiomelanocortin (POMC) gene therapy suppresses melanoma through apoptosis induction and neovascularization blockage. In this study, we investigated the crosstalk between autophagic and apoptotic signaling in POMC-mediated melanoma suppression. By histological and immunoblot analysis, it was shown that POMC-treated melanoma tissues exhibited the prominent LC3 immunostaining, which was correlated with reduced CD31-positive tumor vascularization. Such autophagy induction could be recapitulated in melanoma cells receiving POMC gene delivery and hypoxia-mimicking agent cobalt chloride (CoCl₂). We then utilized the POMC-derived peptide α-MSH with CoCl₂ to elicit the autophagy as

SUBMITTER: Wu JC 

PROVIDER: S-EPMC6060113 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature