Neuronal Calcium and cAMP Cross-Talk Mediated by Cannabinoid CB₁ Receptor and EF-Hand Calcium Sensor Interactions.

ABSTRACT: Endocannabinoids are important players in neural development and function. They act via receptors, whose activation inhibits cAMP production. The aim of the paper was to look for calcium- and cAMP-signaling cross-talk mediated by cannabinoid CB₁ receptors (CB₁R) and to assess the relevance of EF-hand CaM-like calcium sensors in this regard. Using a heterologous expression system, we demonstrated that CB₁R interacts with calneuron-1 and NCS1 but not with caldendrin. Furthermore, interaction motives were identified in both calcium binding proteins and the receptor, and we showed that the first two sensors competed for binding to the receptor in a Ca²⁺-dependent manner. Assays in neuronal primary cultures showed that, CB₁R-NCS1 complexes

SUBMITTER: Angelats E 

PROVIDER: S-EPMC6060245 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature