Resistance to UV Irradiation Caused by Inactivation of nurA and herA Genes in Thermus thermophilus.

ABSTRACT: NurA and HerA are thought to be essential proteins for DNA end resection in archaeal homologous recombination systems. Thermus thermophilus, an extremely thermophilic eubacterium, has proteins that exhibit significant sequence similarity to archaeal NurA and HerA. To unveil the cellular function of NurA and HerA in T. thermophilus, we performed phenotypic analysis of disruptant mutants of nurA and herA with or without DNA-damaging agents. The nurA and herA genes were not essential for survival, and their deletion had no effect on cell growth and genome integrity. Unexpectedly, these disruptants of T. thermophilus showed increased resistance to UV irradiation and mitomycin C treatment. Further, these disruptants and the wild type displayed no

SUBMITTER: Fujii Y 

PROVIDER: S-EPMC6060359 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature