Levels of ADAM10 are reduced in Alzheimer's disease CSF.

ABSTRACT:

Background

The disintegrin metalloproteinase 10 (ADAM10) is the main α-secretase acting in the non-amyloidogenic processing of the amyloid precursor protein. This study assesses whether ADAM10 is present in cerebrospinal fluid (CSF), and whether it has potential as a biomarker for Alzheimer's disease (AD).

Methods

ADAM10 was characterized in human CSF samples by immunoprecipitation and western blotting using antibodies specific for different domains of the protein and by ultracentrifugation in sucrose density gradients. Samples from AD patients (n = 20) and age-matched non-AD controls (n = 20) were characterized for classical CSF biomarkers, Aβ42, T-tau, or P-tau by ELISA, and assayed for soluble ADAM10 levels by western blotting.

Results

We found that ADAM10 is prese

SUBMITTER: Sogorb-Esteve A 

PROVIDER: S-EPMC6060469 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature