Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs.

ABSTRACT: Preservation of hepatic phenotype and functions in vitro has always been a great challenge for the reconstruction of liver tissue engineering and in pharmaceutical research studies. Human induced hepatocytes (hiHeps) generated from fibroblasts can be reproducible with almost normal levels of liver specific functions, which are considered as a new source of hepatocytes for biomedical applications. Moreover, paper has served as an attractive biocompatible material for cell-based applications. In this study, we established a simple paper-based scaffold array for creating a 3D liver co-culture model that enabled the assessment of drug induced hepatotoxicity. The hiHeps co-cultured with HUVECs exhibited a 3D like morphology and maintained the liver specific functions of producing albumin

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6061007 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature