ISEcp1-mediated transposition of chromosome-borne bla_CMY-2 into an endogenous ColE1-like plasmid in Escherichia coli.

ABSTRACT:

Background

CMY-2 is the most prevalent pAmpC β-lactamase, but the chromosomal bla_CMY-2 gene transfer via horizontal transmission has been seldom reported. This study aimed to describe an ISEcp1-mediated transposition of a chromosomal bla_CMY-2 gene from Escherichia coli into a small endogenous ColE1-like plasmid, resulting in elevated resistance to extended-spectrum cephalosporins.

Methods

Three ESCs-resistant ST641 E. coli strains EC6413, EC4103 and EC5106 harbored the bla_CMY-2 gene. S1-PFGE, I-ceu I-PFGE, Southern blotting and electroporation experiments were performed to investigate the location and transferability of bla_CMY-2. The genetic context and gene expression of b

SUBMITTER: Fang LX 

PROVIDER: S-EPMC6061673 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature