SimPhospho: a software tool enabling confident phosphosite assignment.

ABSTRACT: Motivation:Mass spectrometry combined with enrichment strategies for phosphorylated peptides has been successfully employed for two decades to identify sites of phosphorylation. However, unambiguous phosphosite assignment is considered challenging. Given that site-specific phosphorylation events function as different molecular switches, validation of phosphorylation sites is of utmost importance. In our earlier study we developed a method based on simulated phosphopeptide spectral libraries, which enables highly sensitive and accurate phosphosite assignments. To promote more widespread use of this method, we here introduce a software implementation with improved usability and performance. Results:We present SimPhospho, a fast and user-friendly tool for accurate simulation of phosphopeptide

SUBMITTER: Suni V 

PROVIDER: S-EPMC6061695 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature