Supercritical angle Raman microscopy: a surface-sensitive nanoscale technique without field enhancement.

ABSTRACT: Raman scattering microscopy is a versatile tool for label-free imaging and molecular fingerprint analysis. Here, we provide the first demonstration that the selective collection of scattered signals exceeding the critical angle for total internal reflection enables surface-confined spontaneous Raman investigations at nanometre resolution. This high-axial selectivity leads to improved signal-to-background ratios, thus making this technique an excellent probe for surface-related molecular specimens. The richness of the spectroscopic information obtained through the supercritical angle Raman (SAR) collection path was proven by comparing its output with that of a parallel far-field collection path. Furthermore, we demonstrated that the proposed SAR technique is a versatile microscopy approach

SUBMITTER: Serrano D 

PROVIDER: S-EPMC6061911 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature