MiR-146a affects the alteration in myeloid differentiation induced by hydroquinone in human CD34⁺ hematopoietic progenitor cells and HL-60 cells.

ABSTRACT: The MiR-146a/TRAF6/NF-κB axis is important for the regulation of hematopoiesis and the immune system. To identify the key axis that regulates benzene-induced hematotoxicity or even leukemia, we investigated miR-146a expression in human CD34⁺ hematopoietic progenitor cells (HPCs) and human acute promyelocytic leukemia cells (HL-60) during the differentiation process. By performing a colony formation assay and flow cytometry on cells in the differentiation process after hydroquinone treatment, we found that hydroquinone induced a marked reduction of differentiation toward myeloid cells and immune cells in CD34⁺ cells (5 days exposure) as well as in HL-60 cells (3 h exposure). Further study using real-time PCR and western blot showed that the impaired myeloid differentia

SUBMITTER: Yuan W 

PROVIDER: S-EPMC6061933 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature