Ratiometric Tension Probes for Mapping Receptor Forces and Clustering at Intermembrane Junctions.

ABSTRACT: Short-range communication between cells is required for the survival of multicellular organisms. One mechanism of chemical signaling between adjacent cells employs surface displayed ligands and receptors that only bind when two cells make physical contact. Ligand-receptor complexes that form at the cell-cell junction and physically bridge two cells likely experience mechanical forces. A fundamental challenge in this area pertains to mapping the mechanical forces experienced by ligand-receptor complexes within such a fluid intermembrane junction. Herein, we describe the development of ratiometric tension probes for direct imaging of receptor tension, clustering, and lateral transport within a model cell-cell junction. These probes employ two fluorescent reporters that quantify both the liga

SUBMITTER: Ma VP 

PROVIDER: S-EPMC6061938 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature