Defective Early B Cell Tolerance Checkpoints in Sjogren's Syndrome Patients.

ABSTRACT:

Objective

Central and peripheral B cell tolerance checkpoints are defective in many patients with autoimmune diseases, but the functionality of each discrete checkpoint has not been assessed in patients with Sjögren's syndrome (SS). We undertook this study to assess this functionality in SS patients.

Methods

Using a polymerase chain reaction-based approach that allows us to clone and express, in vitro, recombinant antibodies produced by single B cells, we tested the reactivity of recombinant antibodies cloned from single CD19+CD21^low CD10+IgM^high CD27- newly emigrant/transitional B cells and CD19+CD21+CD10-IgM+CD27- mature naive B cells from 5 SS patients.

Results

We found that the frequencies of newly emigrant/transitional B cells expressing pol

SUBMITTER: Glauzy S 

PROVIDER: S-EPMC6062007 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature