Downregulation of catalase by CuO nanoparticles via hypermethylation of CpG island II on the catalase promoter.

ABSTRACT: The advent of nanotechnology has led to new applications of copper as antibiotic treatment alternatives, nanocomposite coatings, catalysts, and lubricants among others. However, few studies address the impact of nano-size copper on the molecular mechanism of eukaryotic cells. Therefore, in the present study, the human hepatic cell line (WRL-68) was used to evaluate the molecular mechanism involved in the adverse effect of CuO NPs. CuO NPs were characterized by scanning electron microscopy and dynamic light scattering to confirm their 100 nm size and their purity was determined by Fourier transform infra-red spectroscopy. The side scattered intensity in WRL-68 cells at a CuO NP concentration of 250, 500, 750 and 1000 μM was found to be 108.83%, 126.86%, 189.03% and 250.88% respectively. The

SUBMITTER: Chibber S 

PROVIDER: S-EPMC6062067 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature