Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations.

ABSTRACT: Monitoring the physicochemical characteristics of nanoparticles following internalisation by cells is a vital step in understanding their biological impact and toxicity. Here, the feasibility of a methodology utilising gentle enzymatic lysis of cells containing internalised particles and direct analysis of the lysates for the particle size, agglomeration state and concentration, is investigated. It is demonstrated that following internalisation, all types of studied silica particles partially agglomerate/aggregate, with the degree and rate of the observed transformation closely correlated with the initial particle surface chemistry. Several different particle populations are noted and characterised in terms of their size and concentration. Good agreement between different complementary tec

SUBMITTER: Bartczak D 

PROVIDER: S-EPMC6062163 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature