Differentiating Positional Isomers of Nucleoside Modifications by Higher-Energy Collisional Dissociation Mass Spectrometry (HCD MS).

ABSTRACT: The analytical identification of positional isomers (e.g., 3-, N⁴-, 5-methylcytidine) within the > 160 different post-transcriptional modifications found in RNA can be challenging. Conventional liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) approaches rely on chromatographic separation for accurate identification because the collision-induced dissociation (CID) mass spectra of these isomers nearly exclusively yield identical nucleobase ions (BH₂⁺) from the same molecular ion (MH⁺). Here, we have explored higher-energy collisional dissociation (HCD) as an alternative fragmentation technique to generate more informative product ions that can be used to differentiate positional isomers. LC-MS/MS of modified nucleosides charac

SUBMITTER: Jora M 

PROVIDER: S-EPMC6062210 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature