Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection.

ABSTRACT: Plasmonic nanoantennas offer new applications in mid-infrared (mid-IR) absorption spectroscopy with ultrasensitive detection of structural signatures of biomolecules, such as proteins, due to their strong resonant near-fields. The amide I fingerprint of a protein contains conformational information that is greatly important for understanding its function in health and disease. Here, we introduce a non-invasive, label-free mid-IR nanoantenna-array sensor for secondary structure identification of nanometer-thin protein layers in aqueous solution by resolving the content of plasmonically enhanced amide I signatures. We successfully detect random coil to cross β-sheet conformational changes associated with α-synuclein protein aggregation, a detrimental process in many neurodegenerative disorde

SUBMITTER: Etezadi D 

PROVIDER: S-EPMC6062318 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature