Gene expression profiling to identify the toxicities and potentially relevant disease outcomes due to endosulfan exposure.

ABSTRACT: Endosulfan, one of the most toxic organochlorine pesticides, belongs to a group of persistent organic pollutants. Gene expression profiling offers a promising approach in health hazard identification of chemicals. The aim of this study was to use gene expression profiling to identify the toxicities and potentially relevant human diseases due to endosulfan exposure. We performed DNA microarray analysis to analyze gene expression profiles in human endothelial cells exposed to 20, 40 and 60 μM endosulfan in combination with an endothelial phenotype. Microarray results showed that endosulfan increased the number of altered genes in a dose-dependent manner, and changed the expression of 161 genes across all treatment groups. qRT-PCR closely matched the microarray data for the genes tested. Sign

SUBMITTER: Xu D 

PROVIDER: S-EPMC6062354 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature