Biphasic modulation of insulin signaling enables highly efficient hematopoietic differentiation from human pluripotent stem cells.

ABSTRACT:

Background

Hematopoietic lineage cells derived from human pluripotent stem cells (hPSCs) hold great promise for the treatment of hematological diseases and providing sufficient cells for immune therapy. However, a simple, cost-effective method to generate large quantities of hematopoietic stem/progenitor cells (HSPCs) is not yet available.

Methods

We established a monolayer, chemically defined culture system to induce hematopoietic differentiation from hPSCs in 8 days.

Results

We found that insulin-free medium allowed hPSCs to leave pluripotency promptly and preferably enter the vascular lineage. Addition of insulin during the later stage of differentiation was essential for the efficient induction of hemogenic endothelium and the emergence of large numbers of CD34

SUBMITTER: Duan F 

PROVIDER: S-EPMC6062919 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature