Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression.

ABSTRACT:

Background

Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we described positive cofactor 4 (PC4) as a protein that contributes to the regulation of replication-dependent histone gene expression.

Results

We showed that PC4 influences RNA polymerase II recruitment to histone gene loci in a cell cycle-dependent manner. The most important effect was observed in S phase where PC4 knockdown leads to the elevated level of RNA polymerase II on histone genes, which corresponds to the increased total level of those gene transcripts. Th

SUBMITTER: Brzek A 

PROVIDER: S-EPMC6062981 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature